Barcelona's 4-2 against Real Betis on Sunday left the Catalans 11 points clear of second-placed Real Madrid.
Messi netted two goals within 15 minutes of coming on as a substitute to give Barcelona a 4-2 comeback win over Real Betis on Sunday.
Madrid's ninth straight home win in league play denied Barcelona the chance to clinch the league title this weekend
Messi damaged a hamstring just over a month ago and missed Champions League tie against Bayern Munich which Barcelona lost.
Barcelona forward Lionel Messi missed training on Friday as he battles to regain fitness for the La Liga leaders' final few matches of the campaign.
Barcelona can secure their 22nd domestic title and their fourth in five years if they beat Real Betis at Camp Nou on Sunday.
Now under the leadership of new coach Tito Vilanova, Barcelona will need to do something to maintain their lofty standards.
Leaders Barcelona were hammered 7-0 on aggregate by Bayern Munich in their semi-final while second-placed Real went down 4-3 against Borussia Dortmund.
Barca suffered their worst ever European defeat over two legs in the last-four clash against Bayern Munich in Champions League semi-finals.
The quality of football played by both the German teams has caught the eye, with their players appearing fitter and stronger.
Barca suffered their worst ever European defeat over two legs in the last-four clash against the German champions.